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| Registros recuperados: 225 | |
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| Garcia, Celine; Francois, Cyrille; Renault, Tristan; Lupo, Coralie. |
| Les 12 et 13 novembre 2013, les journées de la surveillance de la santé des mollusques marins ont rassemblé les acteurs de la santé des mollusques marins (Ifremer, DGAl, DPMA, DDTM, CNC, CRC, laboratoires d'analyses agréés et reconnus, centres techniques). Les objectifs de cette réunion étaient de faire un bilan des activités 2013 du Laboratoire National de Référence (LNR), des réseaux de surveillance (REPAMO) et d'Observation (RESCO et réseaux de centres techniques). |
| Tipo: Text |
Palavras-chave: Réseau Surveillance; Mollusques marins; Maladie; Organismes pathogènes; Détection. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00170/28146/26371.pdf |
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| Le Deuff, Rose-marie; Renault, Tristan. |
| Among the Iridovirus related infections frequently described on many teleosts fish species, Viral Erythrocytic Necrosis (VEN) (Pinto et al., 1989), Epizootic Haematopoietic Necrosis Virus (EHNV) (Langdon et al., 1988) as weil as Lymphocystis Disease Virus (LDV) are not considered to directly cause fatality. Indeed, Lymphocystis outbreaks of farmed fish could be observed in some cases, as a consequence of a bacterial double infection or cannibalism (Ahne ef al., 1991; Moate et al., 1992). Here, we report for the first time a strong LDV infection, and an additional physical stress responsible for mortalities of reared sea bream, Sparus aurata, from Toulon area, on French Mediterranean coast. Although, since the first report of Lymphocystis in sea bream under... |
| Tipo: Text |
Palavras-chave: Mortalities; Sparus aurata; Fish; Iridovirus. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/publication-2886.pdf |
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| Pepin, Jean-francois; Riou, Antoine; Renault, Tristan. |
| Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
| Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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| Lapegue, Sylvie; Renault, Tristan. |
| The most known and certainly the most important contribution of genetics to the oyster indus try is the development of the triploid cupped oyster. Released for the first time in the 1980s, the triploid oyster has triplets of chromosomes instead of pairs. Currently, triploid oysters are obtained by crossing diploid and tetraploid oysters. These oysters are characterized by a near lack of milt in summer, which makes them generally popular with consumers, and by a faster growth, allowing a shorter production cycle. In parallel, the exploitation of natural resistance in the diversity of French oysters has been considered to improve the health of farmed populations which is a major challenge for the sustainability of aquaculture farms. Currently, the... |
| Tipo: Text |
Palavras-chave: Huître creuse; Triploïdie; Sélection; Maladies; Ostréiculture; Cupped oyster; Triploidy; Selection; Disease; Shellfish farming. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60931/64326.pdf |
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| Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter. |
| Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses... |
| Tipo: Text |
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf |
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| Arzul, Isabelle; Renault, Tristan. |
| In 1972, Farley reported the presence of herpes-type viral particles in adult American oysters (Crassostrea virginica) for the first time. Since then, this pathogen has been detected in various marine bivalve species, usually associated with massive mortality events. To date, herpes-type viruses have been detected in the following species: C gigas, Ostrea edulis, O. angasi, Tiostrea chilensis, Ruditapes decussatus, R. philippinarum and most recently, Pecten maximus. |
| Tipo: Text |
Palavras-chave: Pathogènes; Bivalves; Herpesvirus; Herpesviridae. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3266.pdf |
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| Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan. |
| Background: Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. Results: The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant... |
| Tipo: Text |
Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf |
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| Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Chollet, Bruno; Cochennec, Nathalie; Renault, Tristan. |
| Le réseau REPAMO répond à plusieurs objectifs, définis dans différentes Directives Européennes: prévenir et détecter l'introduction et la propagation d'un agent pathogène émergent, réémergent ou exotique pour les productions de coquillages d'intérêt commercial en France, surveiller l'évolution des maladies à déclaration obligatoire, surveiller les échanges avec nos partenaires commerciaux. Le fonctionnement repose sur la collaboration entre les professionnels, la DPMCM, les laboratoires côtiers d'IFREMER, et trois cellules d'analyses. Les prélèvements et les outils diagnostiques choisis dépendent des différents objectifs. Le suivi est fait sur des populations sauvages et cultivées du littoral français. Le nombre d'analyses progresse régulièrement grâce à... |
| Tipo: Text |
Palavras-chave: Réseau surveillance; Pathologie; Mortalités; Mollusques marins. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00098/20904/18523.pdf |
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| Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan. |
| Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted... |
| Tipo: Text |
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf |
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| Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre. |
| Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of... |
| Tipo: Text |
Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf |
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| Renault, Tristan; Faury, Nicole; Barbosa Solomieu, Valérie; Moreau, Kevin. |
| Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the... |
| Tipo: Text |
Palavras-chave: Pacific cupped oyster; Crassostrea gigas; OsHV-1; Immunity; Gene expression; Multicopper oxidase; Macrophage expressed protein; Ig cell adhesion molecule; Interferon-induced protein 44; Virus RNA. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15466/12843.pdf |
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| Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Arzul, Isabelle; Robert, Maeva; Omnes, Emmanuelle; Chollet, Bruno; Renault, Tristan. |
| The Ifremer Repamo network (REseau de PAthologie des MOllusques) is the national surveillance network in charge of monitoring marine mollusc health on French coastlines. It is commissioned to enforce the official regulations and to provide a public service, under the supervision of the competent authority (DGAL Veterinary Directorate) in the Ministry.of Agriculture and Fisheries. Its goals are to prevent the introduction and the dissemination of infectious agents, in particular pathogens involved in reportable diseases, and to monitor the changes in the pathogens already present on the French coasts. |
| Tipo: Text |
Palavras-chave: Network; Surveillance; Mollusks; Pathogens; Regulations. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6946.pdf |
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| Renault, Tristan. |
| Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most... |
| Tipo: Text |
Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf |
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| Renault, Tristan. |
| Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les outils de diagnostic développés sont basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR). Ce projet, proposé sur une durée de trois années (1995-1998),... |
| Tipo: Text |
Palavras-chave: Virus; Pathologie; Herpès virus; Crassostrea gigas; Pathogène; Herpesviridae. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00043/15450/12825.pdf |
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| Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan. |
| Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and... |
| Tipo: Text |
Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf |
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| Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
| The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
| Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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| Registros recuperados: 225 | |
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